Movement of Cucumber Mosaic Virus Is Restricted at the Interface between Mesophyll and Phloem Pathway in Cucumis figarei
スポンサーリンク
概要
- 論文の詳細を見る
Viral movement in the leaf tissues of a resistant host, Cucumis figarei, inoculated with the pepo strain of Cucumber mosaic virus(CMV)and incubated at 24°C or 36°C was investigated by fluorescence in situ hybridization(FISH), leaf-press blotting, tissue printing and immunogold-silver staining techniques. Observation by FISH revealed that at 24°C most infection sites with CMV at 0.01 mg / ml or 0.1 mg / ml were limited to a single cell during the incubation period, that the number of infection sites increased from 24 hpi(hours post inoculation)to 80 hpi in the leaves inoculated with CMV at 0.5 mg / ml, and that the size as well as the number of infection sites rapidly increased with time in the leaves inoculated with CMV at 2.0 mg / ml. These results suggested that one factor for the resistance of C.figarei at 24°C might be an inhibition of viral movement in and out of the infection sites. Leaf-press blotting and tissue blotting indicated that CMV remained in the infection sites at 24°C, whereas it spread from the inoculated leaves to other parts of the plants through vascular systems at 36°C. Immunogold-silver staining demonstrated that at 24°C CMV infected bundle shesth(BS)cells in minor veins, whereas at 36°C it invaded not only BS cells, but also phloem parenchyma(PP) / companion cell(CC)or PP / intermediary cell(IC)complexes in minor veins in the regions with chorotic symptoms. Tese results indicated that at 24°C CMV had difficulty in passing through the interface between BS and PP / CC or PP / IC complexes and that viral entry from mesophyll to the phloem pathway was inhibited in the inoculated leaves.
- 日本植物病理学会の論文
- 2000-05-25
著者
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Ohki Satoshi
Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University
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Ohki S
Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University
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KOBORI Takashi
Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
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OSAKI Takeshi
Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
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Osaki T
Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University
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Osaki Takeshi
Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University
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Kobori Takashi
Graduate School Of Agriculture And Biological Sciences Osaka Prefecture University
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OHKI Satoshi
Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
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