Hydrolysis of Palm Stearin Oil by a Thermostable Lipase in a Draft Tube-Type Reactor

概要

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Palm stearin oil, which has a high melting point and is generaly used as the commercial raw material in oil chemistries, was hydrolyzed by a thermostable lipase produced from Pseudomonas sp. The initial reaction rate of oil hydrolysis was controlled by the interfacial area of the water/oil emulsion (W/O emulsion) where water was emulsified in oil, and it was represented by the Michaelis-Menten equation in which the interfacial area was substitute for the substrate concentratin. A new bioreactor of a draft tube type which has the capability of functioning as an efficient continuous reactor for the separation of oil and water and the recovery of the enzyme was developed. By using the single-stage reactor, the hydrolysis ratio of palm stearin oil was maintained at 80% up to 230 h of operation, and percentage recovery of the enzyme was 98.8%. A countercurrent multistage reactor (three-stage reactor) as developed with athe aim of improving the hydrolysis ratio. In the continuous hydrolysis of palm stearin oil using this reactor, the retention time of which was 36 h at ech reactor, a high percentage hydrolysis of 95% and an increased glycerin concentration of up to 30% were observed.
公益社団法人日本生物工学会の論文
1995-10-25