Hydrolysis of Soybean Oil by Lipase with a Bioreactor Having Two Different Membranes

概要

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A membrane bioreactor with 25 sets of two different membranes, one hydrophilic and the other hydrophobic microporous, was used for the hydrolysis of soybean oil by lipase frome Candida cylindracea. In the bioreactor, oil, water and enzyme liquid were separately fed into each of 3 clearances between the two membranes. Oil diffused through the hydrophobic membrane and water diffused through the hydrophilic membrane came into contact with the lipase, and hydrolysis subsequently took place. Fatty acid and glycerine produced by the enzymatic reaction diffused through each membrane into the oil and water sides respectively, so that these products were separated from each other. The enzyme was also separated from the products. In this membrane bioreactor, both reaction and separation occurred. Semi-continuous reaction of soybean oil with one cycle of 48 h was carried out for 18 d, at 45℃. Satisfactory performance of the bioreactor, such as a high hydrolysis percentage (92-88%) and enzyme stability over a long-term period was shown. Enzyme stability was found to depend on produced glycerine, as observed by Yamane et al., and the rate of the hydrolysis reaction was controlled by that of the diffusion of fatty acid through the hydrophobic membrane.
社団法人日本生物工学会の論文
1993-01-25