A Large Plasmid Isolated from Rhizobium huakuii bv. renge That Includes Genes for Both Nodulation of Astragalus sinicus cv. Japan and Nitrogen Fixation

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概要

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• Rhizobium huakuii bv. renge, which is able to produce nodules on Astragalus sinicus cv. Japan, has been assigned to a new biovarlety based on its host range and taxonomic characteristics. We reproducibly demonstrated the presence of an indigenous large plasmid, with a molecular mass of about 260 Mda, in R. huakuii bv. renge and named the plasmid pRhYM. Using a heat-curing procedure, we obtained deribative strains of R. huakuii bv. renge that were defective for nodulation (Nod^-) and nitrogen fixation (Fix^-). Nod^- mutant strains either did not contain pRhYM or had deletions of pRhYM segments. [^<32>P]-Labeled probes of cloned DNA fragments of nod and nif from Rhizobium sp. HGR234 and Klebstella pneumoniae, respectively, hybridized to pRhYM of R.huakuii bv. renge, but not to DNA from most of the Nod^- mutant strains. These results show that the nod and nif genes of R. huakuii bv. renge are located on pRhYM.

• 公益社団法人日本生物工学会の論文
• 1995-09-25

著者

• Xu Yong

  Department Of Fermentation Technology Faculty Of Engineering Hiroshima University

• MUROOKA YOSHIKATSU

  (Present address)Department of Fermentation Technology, Faculty of Engineering, Hiroshima University

• Murooka Yoshikatsu

  (present Address)department Of Biotechnology Faculty Of Engineering Osaka University

関連論文

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• A Large Plasmid Isolated from Rhizobium huakuii bv. renge That Includes Genes for Both Nodulation of Astragalus sinicus cv. Japan and Nitrogen Fixation

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