On-Line Continuous Measurement of the Oxygen Consumption Rate in Mammalian Cell Culture

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概要

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• A method for on-line, continuous measurement of the oxygen consumption rate (Io_2) by mammalian cells on a microcarrier was developed and its reliability investigated. Utilizing the periodic dissolved oxygen (DO) fluctuation in the normal on-off control of DO, on-line, continuous measurement of Io_2 was carried out in which real-time estimation of the DO saturation concentration was made by measuring the gas-phase pressure and the gas-phase oxygen concentration. It was found that the continuously measured Io_2 value was quantitatively exact and could be applied commerically using the oxidation reaction of glucose by glucose oxidase.

• 社団法人日本生物工学会の論文
• 1994-06-25

著者

• Takagi Mutsumi

  Life Science Research Laboratories Asahi Chemical Industry Company Ltd.

• UEDA KAZUO

  Life Science Research Laboratories, Asahi Chemical Industry Company Ltd.,

• Ueda Kazuo

  Life Science Research Laboratories Asahi Chemical Industry Company Ltd.

関連論文

• Effect of Insoluble Medium Component on the Productivity of Tissue Plasminogen Activator by Cells on Microcarriers
• An Oxygen Supply Strategy for the Large-Scale Production of Tissue Plasminogen Activator by Microcarrier Cell Culture
• A Low-Serum Medium with BSA and Ferrous Sulfate for the Production of Tissue Plasminogen Activator by Human Embryo Lung Cells
• On-Line Continuous Measurement of the Oxygen Consumption Rate in Mammalian Cell Culture
• On-Line Determination of Optimum Time for Switching from Growth Phase to Production Phase in Tissue Plasminogen Activator Production in a Microcarrier Cell Culture

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