Nucleotide Sequence of Gene celM Encoding a New Endoglucanase (CelM) of Clostridium thermocellum and Purification of the Enzyme

スポンサーリンク

概要

• 論文の詳細を見る

• The nucleotide sequence of a new endoglucanase gene (celM) of Clostridium thermocellum was determined. The structural gene contains an open reading frame of 978 bp starting with an ATG codon and terminating in a TGA stop codon. The deduced amino acid sequence of endoglucanase M (CelM) contains 325 amino acids (MW 35,186) and its N-terminal amino acid sequence is identical to that of the purified enzyme. The codon usage of the gene is similar to that of other endoglucanase genes in the same organism. The endoglucanase was purified from Escherichia coli with a 200-fold increase in specific acitivity. The optimum pH and temperature values of the enzyme are 5.5 to 6.5 and 60℃ respectively for carboxymethylcellulose (CMC) degradation.

• 社団法人日本生物工学会の論文
• 1993-10-25

著者

• DEMAIN Arnold

  Fermentation Microbiology Laboratory, Massachusetts Institute of Technology

• Demain Arnold

  Fermentation Microbiology Laboratory Department Of Biology Massachusetts Institute Of Technology Cam

• Demain Arnold

  Fermentation Microbiology Laboratory Biology Department Massachusetts Institute Of Technology

• KOBAYASHI TOHRU

  Fermentation Microbiology Laboratory, Department of Biology, Massachusetts Institute of Technology,

• ROMANIEC MAREK

  Fermentation Microbiology Laboratory, Department of Biology, Massachusetts Institute of Technology,

• BARKER PATRICK

  Institute of Animal Physiology and Genetics Research, Babraham Hall

• GERNGROSS ULF

  Fermentation Microbiology Laboratory, Department of Biology, Massachusetts Institute of Technology,

• Gerngross Ulf

  Fermentation Microbiology Laboratory Department Of Biology Massachusetts Institute Of Technology Cam

• Romaniec Marek

  Fermentation Microbiology Laboratory Department Of Biology Massachusetts Institute Of Technology Cam

• Barker Patrick

  Institute Of Animal Physiology And Genetics Research Babraham Hall

• Kobayashi Tohru

  Fermentation Microbiology Laboratory Department Of Biology Massachusetts Institute Of Technology Cam

関連論文

• Mutagenesis of the Rapamycin Producer Streptomyces hygroscopicus FC904
• Enhancement of the Antifungal Activity of Rapamycin by the Coproduced Elaiophylin and Nigericin
• New Microorganisms which Convert Compactin to Pravastatin
• Glutamine Dose Not Repress Gramicidin S Biosynthesis in Bacillus brevis
• Nucleotide Sequence of Gene celM Encoding a New Endoglucanase (CelM) of Clostridium thermocellum and Purification of the Enzyme
• Biotransformation of Compactin to Pravastatin by Actinomadura sp. 2966
• Antifungal Activities of Rapamycin and Its Derivatives, Prolylrapamycin, 32-Desmethylrapamycin, and 32-Desmethoxyrapamycin

スポンサーリンク