A Method for Increasing the Success Rate of Duplicating Antibiotic Activity in Agar and Liquid Cultures of Streptomyces Isolates in New Antibiotic Screens

概要

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During screens for novel secondary metabolite natural products, actinomycete isolates are often encountered which show antibiotic activity on agar but not in liquid culture. In this study, it was found that the liquid culture morphology of Streptomyces species, producing antibiotic on solid media only, was fragmented compared with that of the filamentous mycelia of antibiotic-producing Streptomyces. An enrichment culture method which selected for a more filamentous liquid culture morphology was successful in providing active liquid cultures of the isolates which had previously been active only in agar culture. After repeating the selection procedure five times with several cultures, two stable phenotypes with antibiotic activity in liquid culture and a filamentous morphology were obtained. The correlation of a filamentous morphology with antibiotic production in liquid culture is discussed.
公益社団法人日本生物工学会の論文
1993-08-25