Genetic Recombination by Spheroplast Fusion of Zymomonas Auxotrophs

スポンサーリンク

概要

• 論文の詳細を見る

• Auxotrophic mutants were isolated from Zymomonas mobilis NRRL B-14023 by NTG treatment. Eleven out of 18 auxotrophs that turned into spheroplasts in a culture medium containing glycine and/or penicillin could be regenerated into intact cells with the order of 1-10% in frequency. By using auxotrophy as selective markers, prototrophic fusants were obtained with a frequency of the order of 10^<-6> per number of progeny regenerated. The fusants obtained were confirmed from the segregation test to be genetic recombinants.

• 社団法人日本生物工学会の論文
• 1989-10-25

著者

• Yoshida Y

  Res. Dev. Corp. Japan Saitama Jpn

• YOSHIDA YOSHINORI

  Central Research Laboratories, Mercian Corporation

• SUZUKI RINA

  Central Research Laboratories, Mercian Corporation

• YAGI YOSHIAKI

  Central Research Laboratories, Mercian Corporation

• TAIRA KEIJIRO

  Central Research Laboratories, Sanraku Incorporated

• Yoshida Yohko

  Research Institute Takeya Miso Co. Ltd.

• Suzuki Rina

  Central Research Laboratories Mercian Corporation

• Taira Keijiro

  Central Research Laboratories Sanraku Incorporated

関連論文

• Production of Levan by a Zymomonas sp.
• Genetic Recombination by Spheroplast Fusion of Zymomonas Auxotrophs
• Lipase Production of Aspergillus oryzae
• Physiological Properties of Two Pseudomonas mendocina Strains Which Assimilate Styrene in a Two-Phase (Solvent-Aqueous) System under Static Culture Conditions
• Production of 3-Vinylcatechol and Physiological Properties of Pseudomonas LF-3, Which Can Assimilate Styrene in a Two-phase (Solvent-Aqueous) System
• Purification and Characterization of a Novel Lipolytic Enzyme from Aspergillus oryzae
• Effect of an External Magnetic Flux on Antitumor Antibiotic Neocarzinostatin Yield by Streptomyces carzinostaticus var. F-41
• Improved Production of a Chromophore Component of Antitumor Antibiotic Neocarzinostatin (NCS) by Addition of Threonine in a Chemically Defined Medium
• Determination of CGTase from Bacillus ohbensis and Its Optimum pH Using HPLC

スポンサーリンク