Efficient Synthesis of O-Linked Glycopeptide by a Transglycosylation Using Endo α-N-Acetylgalactosaminidase from Streptomyces sp.

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概要

• 論文の詳細を見る

• Galβ-(1→3)-GalNAc-linked hexapeptide was synthesized by a transglycosylation using Galβ-(1→3)-Gal-NAcβ-pNP as a donor and a serine-containing hexapeptide as an acceptor using endo GalNAc-ase from Streptomyces sp.. The Galβ-(1→3)-GalNAc residue was transferred to the hydroxyl group of the serine residue of the peptide. The total yield of the glycopeptide via this process was better than that of the chemoenzymatic method. This process was confirmed to be a versatile method for the synthesis of O-linked glycopeptides.

• 社団法人日本農芸化学会の論文
• 2001-05-23

著者

• Ajisaka Katsumi

  Nutrition Science Institute Meiji Milk Products Co.ltd.

• Ajisaka Katsumi

  Nutrition Science Institute Meiji Milk Products Co. Ltd.

• Ishii‐karakasa I

  Department Of Biochemistry School Of Medicine Kitasato University

• ISHII-KARAKASA Ikuko

  Department of Biochemistry and Medicine, School of Medicine and Nursing, Kitasato University

• MIYASATO Mariko

  Nutrition Science Institute, Meiji Milk Products Co.Ltd.

• Miyasato Mariko

  Nutrition Science Institute Meiji Milk Products Co.ltd.

関連論文

• Estimation of the Number of O-Linked Oligosaccharides per Heavy Chain of Human Serum IgAl by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOFMS) Analysis of the Hinge Glycopeptide
• Abundance of Galβ1,3GalNAc in O-Linked Oligosaccharide on Hinge Region of Polymerized IgA1 and Heat-Aggregated IgA1 from Normal Human Serum
• Efficient Synthesis of O-Linked Glycopeptide by a Transglycosylation Using Endo α-N-Acetylgalactosaminidase from Streptomyces sp.
• ケモエンザイマティックな手法による糖タンパク質糖鎖および糖鎖付加ペプチドの効率的合成
• Convenient Analytical Methods for Endo-type Glycosidase that Acts on Glycoconjugates and Their Application in Glycotechnology

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