Purification, Characterization and Substrate Specificities of Xylanase Isoenzymes from Melanocarpus albomyces IIS 68
スポンサーリンク
概要
- 論文の詳細を見る
The ascomycetous fungus Melanocarpus albomyces when grown on wheat straw produced seven extracellular xylanase isoenzymes, designated as Ia, Ib, Ic, IIa, IIb, IIc, and IId. All seven xylanses were purified to homogeneity by gel filtration and ion-exchange chromatography. The molecular mass (kDa) of Ia, Ib, Ic, IIa, IIb, IIc, and IId were estimated to be 22.9,20.7,18.6,31.3,25.4,38.5,and 34.3,respectively by SDS-PAGE, and 23.7,20.5,17.1,31.7,25.1,39.8,and 32.2,respectively by gel filtration. The isoelectric points of Ia, Ib, Ic, IIa, and IIb were found to be 6.2,6.3,6.4,3.7,and 4.4,respectively. The activity of the isoenzymes was dependent on the type of the xylan substrates; Xylanases Ia, Ib, and Ic showed highest specific activity toward larchwood xylan (an arabinoglucuronoxylan), IIa an IIc toward birchwood xylan (a glucuronoxylan), and IIb and IId toward beechwood xylan (a glucuronoxylan). Four isoenzymes Ia, Ib, Ic, and IIa had an arabinose-releasing property on larchwood xylan. Application of specific isoenzymes as prebleaching agents in paper manufacture is proposed.
- 社団法人日本農芸化学会の論文
- 2000-06-23
著者
-
Bisaria Virendra
Department Of Biochemical Engineering & Biotechnology Indian Institute Of Technology
-
Saraswat V
Department Of Biochemical Engineering And Biotechnology Indian Institute Of Technology
-
Saraswat Vibhor
Department Of Biochemical Engineering And Biotechnology Indian Institute Of Technology
関連論文
- Bioprocess strategies for enhanced production of xylanase by Melanocarpus albomyces IITD3A on agro-residual extract(BIOCHEMICAL ENGINEERING)
- Optimization of Xylanase Production by Melanocarpus albomyces IIS68 in Solid State Fermentation Using Response Surface Methodology
- Production of Podophyllotoxin by Plant Cell Cultures of Podophyllum hexandrum in Bioreactor
- Exogenous Hormones Affecting Morphology and Biosynthetic Potential of Hairy Root Line(LYR2i) of Linum album(PLANT BIOTECHNOLOGY)
- Adsorption Characteristics of Cellulases from a Constitutive Mutant of Trichoderma reesei
- Purification, Characterization and Substrate Specificities of Xylanase Isoenzymes from Melanocarpus albomyces IIS 68
- Enhanced withanolide production by overexpression of squalene synthase in Withania somnifera(PLANT BIOTECHNOLOGY)
- Enhanced withanolide production by overexpression of squalene synthase in Withania somnifera