Two-mode Analysis by High-performance Liquid Chromatography of ρ-Aminobenzoic Ethyl Ester-derivatized Monosaccharides

スポンサーリンク

概要

• 論文の詳細を見る

• ρ-Aminobenzoic ethyl ester (ABEE)-derivatized monosaccharides were separated by HPLC with a trifluoroacetic acid (TFA) solution or borate buffer as the eluent. In the case of the TFA solution, ABEE-derivatized monosaccharides of the neutral and amino sugars found in animal glycoproteins were separated in a simultaneous analysis. In the case of the borate buffer, ABEE-derivatized monosaccharides of identical molecular weights such as ABEE-Gal, -Glc, and -Man were separated as stereoisomers. Glucuronic acid and galacturonic acid were detected and separated within 8 min. The relationship between the peak areas and the amounts of ABEE-derivatized monosaccharides was linear in the range of 1 to 1000 pmol.

• 社団法人日本農芸化学会の論文
• 1997-11-23

著者

• Kamei Masugu

  Sugiyama Chemical And Industrial Laboratory

• Murata Takeomi

  Honen Corporation

• Kamei Masaharu

  Sugiyama Chemical And Industrial Laboratory

• KOKUBO Kazuko

  Honen Corporaton

• YASUNO Shoichi

  Sugiyama Chemical and Industrial Laboratory

• YAMAGUCHI Takashi

  Honen Corporation

関連論文

• New Method for Determining the Sugar Composition of Glycoproteins, Glycolipids, and Oligosaccharides by High-performance Liquid Chromatography
• Two-mode Analysis by High-performance Liquid Chromatography of ρ-Aminobenzoic Ethyl Ester-derivatized Monosaccharides
• Cytotoxicity of Cholestane 3β,5α,6β-Triol on Cultured Intestinal Epithelial Crypt Cells (IEC-6)
• Cytotoxicity of 7-Ketocholesterol toward Cultured Rat Hepatocytes and the Effect of Vitamin E
• Laboratory and Field Evaluation of Methoprene and Its Slow-Release Formulation, Altosid^ 10F, against the Sprinkler Sewage Filter Fly, Psychoda alternata SAY(Diptera:Psychodidae)

スポンサーリンク