Nitroguanidination of the Amino Groups of Ribonuclease T_1 with N-Methyl-N'-nitro-N-nitrosoguanidine
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概要
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Ribonuclease T_1 was nitroguanidinated with N-methyl-N'-nitro-N-nitrosoguanidine to investigate the effects of modification of the amino groups on the activity and at the same time to shed some light on its usefulness in specific chemical modification of proteins. Under the conditions used (at pH 7.0 and 37℃ for 72h), the amino group of the NH_2-terminal Ala 1 was modified completely, and the ε-amino group of Lys 41 about 90%. No other residue was modified. The modified enzyme had approximately 84% of the original activity toward RNA, and showed a characteristic absorption maximum at 270 nm.
- 社団法人日本農芸化学会の論文
- 1997-09-23
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