Galactosyl Transfer onto p-Nitrophenyl β-D-Glucoside Using β-D-Galactosidase from Bacillus circulans

概要

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β-D-Gal-(1→4)-β-D-Glc-OC_6H_4NO_2-p and its isomers (β-D-Gal-(1→3)-β-D-Glc-OC_6H_4NO_2-p and β-D-Gal-(1→6)-β-D-Glc-OC_6H_4NO_2-p) were synthesized from lactose and β-D-Glc-OC_6H_4NO_2-p, using transglycosylation by the β-D-galactosidase from Bacillus circulans. This reaction was efficient enough for us to do a one-pot preparation of galactosyl-glucoside from lactose. The order of the production of the transfer products was (1→4) ≫ (1→3) > (1→6) in the initial stage of the reaction, and the same relationship was observed for the hydrolytic rate toward the three galactosyl-glucosides. The production of (1→4)- and (1→3)-linkages greatly decreased during the subsequent reaction and much more of the (1→6)- than of the (1→4)- and (1→3)-transfer products was found in the later stage of the reaction.
社団法人日本農芸化学会の論文
1997-07-23