Mercuric Ion Uptake by Escherichia coli Cells Producing Thiobacillus ferrooxidans MerC

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概要

• 論文の詳細を見る

• The merC gene of Thiobacillus ferrooxidans was overexpressed in Escherichia coli under the control of the tac promoter. MerC protein synthesized in E. coli has a N-terminal amino acid sequence of S-A-I-X-R-I-I-D-K-I-G-I-V-G-, which agrees with the amino acid sequence deduced from its nucleotide sequence except that an initiating methionine residue was removed. The MerC protein was localized in the particulate (membrane) cell fraction, and not in the soluble cytoplasmic fraction. E. coli cells carrying a plasmid containing the tac promoter-directed merC showed ^<203>Hg^<2+> uptake in an isopropyl-1-thio-β-D-galactopyranoside (IPTG)-dependent manner.

• 社団法人日本農芸化学会の論文
• 1996-08-23

著者

• Kusano Tomonobu

  Central Research Laboratory Dowa Mining Co. Ltd

• Inoue Chihiro

  Laboratory Of Plant Genetic Engineering Biotechnology Institute Akita Prefectural College Of Agricul

• Silver Simon

  Department Of Microbiology And Immunology M/c 790 University Of Illinois

• Silver Simon

  Department of Microbiology and Immunology, University of Illinois at Chicago

関連論文

• The Bacterial View of the Periodic Table:Specific Functions for All Elements (Mini-Reviews特集「微生物生態と環境バイオテクノロジ-2」)
• Interactions between Two MerR Regulators and Three Operator/Promoter Regions in the Mercury Resistance Module of Bacillus megaterium
• Functional Dissection of a Mercuric Ion Transporter, MerC, from Acidithiobacillus ferrooxidans
• Mercuric Ion Uptake by Escherichia coli Cells Producing Thiobacillus ferrooxidans MerC

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