Two Forms of α-Glucosidase from Mucor javanicus Produced by Culture with Castanospermine

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概要

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• When Mucor javanicus was cultured with castanospermine, two α-glucosidases (CS-I, II) were produced. The two enzymes were purified by two CM-cellulofine column chromatographies, and compared with the α-glucosidase (C) from a culture without castanospermine. The molecular weights of the three enzymes were calculated to be 97,000 (CS-I, II) and 94,000 (C) from SDS-PAGE. CS-II was similar in hydrolysis of α-1,4-linkage to C, but hydrolyzed α-1,2-, α-1,3-, and α-1,6-linkages at a lower rate than C. The Michaelis constant of CS-I for malto-oligosaccharides was several times as high as the constants of CS-II and C, but CS-I hydrolyzed nigerose at a faster rate than maltose.

• 社団法人日本農芸化学会の論文
• 1996-03-23

著者

• Konno Haruyoshi

  Research Institute For Bioresources Okayama University

• Yamasaki Yoshiki

  Research Institute For Bioresources Okayama University

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