Two-dimensional Gel Electrophoresis of Ribosomal Proteins as a Novel Approach to Bacterial Taxonomy : Application to the Genus Arthrobacter
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概要
- 論文の詳細を見る
Ribosomal proteins from 22 strains of 15 different species belong to the genus Arthrobacter were analyzed by an improved two-dimensional gel electrophoresis. Electrophoretograms of ribosomal proteins from 15 type strains had species-specific patterns. Similarity coefficients (S_<AB> values) of ribosomal proteins with mol. wt. of greater than about 20, 000, among strains of the same species (DNA relatedness values of more than 61%) were greater than 0. 85, but the S_<AB> values among strains of different species were less than 0. 60. The N-terminal amino acid sequences of the AL2 proteins, which migrated into similar positions in this method, from 5 type strains were shown to be highly homologous. Our results indicated that ribosomal proteins have been conserved within species during evolution and that the members of the genus Arthrobacter are phylogenetically homogeneous. Thus, ribosomal protein profiles by this method are a potential tool for strain identification.
- 社団法人日本農芸化学会の論文
- 1995-09-23
著者
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OCHIAI KEIKO
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Kawamoto Isao
Research Laboratories Sankyo Co. Ltd
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Kawamoto Isao
Research Laboratories Sankyo Co. Ltd
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Kawamoto Isao
Medicinal Chemistry Research Laboratories Sankyo Co. Ltd.
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Kawamoto Isao
Research Laboratories Sankyo Co. Ltd.
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Kawamoto Isao
Tsukuba Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Ochiai K
Kyowa Hakko Kogyo Co. Ltd. Tokyo Jpn
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Ochiai Keiko
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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