Construction of Pta-Ack Pathway Deletion Mutants of Escherichia coli and Characteristic Growth Profiles of the Mutants in a Rich Medium

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概要

• 論文の詳細を見る

• Escherichia coli grown in a rich medium excreted acetate and reused the acetate. Using cloned genes and a plasmid with a temperature-sensitive replication origin, three kinds of Pta-Ack pathway deletion mutants were constructed. Acetate production and reuse by wild-type cells grown in the rich medium was confirmed to largely occur through the Pta-Ack pathway. The deletion mutants of the gene encoding phosphotransacetylase secreted pyruvate before the secretion of acetate into the medium. A deletion mutant of the gene endocing acetate kinase grew at a slow rate, but its secretion and use of acetate were rapid. These results indicated that a pathway(s), other than the Pta-Ack pathway, functions in the control of excess carbon flow in the mutants.

• 社団法人日本農芸化学会の論文
• 1994-12-23

著者

• Hosono Kiyoshi

  Laboratory Of Alicrobiology School Of Agriculture Nagoya University

• 角田 浩之

  九州大学農学部動物学教室

• 角田 浩之

  福岡南女子高等学校

• Kakuda Hiroyuki

  Laboratory of Alicrobiology, School of Agriculture, Nagoya University

• Ichihara Shigeyuki

  Laboratory of Alicrobiology, School of Agriculture, Nagoya University

• Shiroishi Kazuya

  Laboratory of Microbiology, School of Agriculture, Nagoya University

• Ichihara Shigeyuki

  Laboratory Of Alicrobiology School Of Agriculture Nagoya University

• Ichihara Shigeyuki

  Department Of Applied Biological Science Faculty Of Agriculture Nagoya University

• Kakuda H

  Mitsukan Group Corp. Aichi Jpn

• Shiroishi Kazuya

  Laboratory Of Microbiology School Of Agriculture Nagoya University

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