Depolymerization of Hyaluronic Acid by D-Fructose 6-Phosphate
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概要
- 論文の詳細を見る
Depolymerization of hyaluronic acid obtained from Streptococcus zooepidemicus by D-fructose 6-phosphate was investigated for characterization of reducing sugar-mediated degradation of biopolymers under physiological conditions. The extent of depolymerization was monitored by the decrease of viscosity of a reaction mixture containing 1.0% hyaluronic acid, D-fructose 6-phosphate, and 1.0×10^<-2> mM of Cu^<2+> in phosphate buffer, pH 7.4. It was found that the depolymerization of hyaluronic acid was dependent on the concentration of the reducing sugar and was specifically accelerated by the presence of Cu^<2+>. The reaction was found to be significantly inhibited by catalase, superoxide dismutase (SOD), 1,2-dihydroxybenzene 3,5-disulfonic acid (Tiron), and chelating agents such as EDTA and diethylene triamine penta-acetic acid (DETAPAC), although the inhibition by SOD was low. Almost the same depolymerization rates were observed in hyaluronic acid preparations of different molecular weight (1.1×10^6, 8.8×10^5, and 6.8×10^5). The rates, however, were different for hyaluronic acids obtained from S. zooepidemicus, rooster comb, and umbilical cord. It was concluded that depolymerization of the polysaccharide was caused by active oxygen species generated by the autoxidation of D-fructose 6-phosphate in the presence of Cu^<2+>, in a mechanism similar to that previously reported for the degradation of DNA and inactivation of virus in vitro.
- 社団法人日本農芸化学会の論文
- 1993-12-23
著者
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NISHIKAWA Shiro
Department of Life Science, Faculty of Bioresources, Mie University
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KASHIMURA Naoki
Department of Life Science, Faculty of Bioresources, Mie University
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Sato I
Chisso Corp. Yokohama Jpn
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Nishikawa Shiro
Department Of Life Science Faculty Of Bioresources Mie University
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Kashimura N
Department Of Life Science Faculty Of Bioresources Mie University
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Zu Jian
Laboratory of Biofunctional Chemistry, Department of Bioscience, Faculty of Bioresources, Mie Univer
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Nishikawa Shiro
Laboratory of Biofunctional Chemistry, Department of Bioscience, Faculty of Bioresources, Mie Univer
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Kashimura Naoki
Laboratory of Biofunctional Chemistry, Department of Bioscience, Faculty of Bioresources, Mie Univer
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Sato Ikuo
Laboratory of Biofunctional Chemistry, Department of Bioscience, Faculty of Bioresources, Mie Univer
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Zu Jian
Laboratory Of Biofunctional Chemistry Department Of Bioscience Faculty Of Bioresources Mie Universit
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