Physical Mapping of Multiple Homologous Genes in the Bacillus subtilis 168 Chromosome : Identification of Ten Ribosomal RNA Operon Loci

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概要

• 論文の詳細を見る

• Ten ribosomal RNA operons (rrn) of the Bacillus subtilis 168 strain were precisely located in the complete physical map, i. e. , the NotI and SfiI restriction map [M. Itaya and T. Tanaka, J Mol. Biol. , 220, 631-648 (1991)]. Mapping was done by identification of the NotI and the SfiI fragments to which the rrnO operon hybridized. This protocol proved that homologous multiple genes dispersed on the chromosome can be physically mapped by using a single membrane filter. A previously unidentified deletion formation was shown to be that caused by homologous recombination between rrnJ-W.

• 社団法人日本農芸化学会の論文
• 1993-09-23

著者

• Itaya M

  Mitsubishi Kagaku Inst. Life Sci. Tokyo Jpn

• Itaya Mitsuhiro

  Nitsubishi Kasei Institute of Life Sciences

関連論文

• Stable Positional Cloning of Long Continuous DNA in the Bacillus subtilis Genome Vector
• Efficient Cloning and Engineering of Giant DNAs in a Novel Bacillus subtilis Genome Vector
• Physical Mapping of Multiple Homologous Genes in the Bacillus subtilis 168 Chromosome : Identification of Ten Ribosomal RNA Operon Loci

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