Isolation and Characterization of a Sialic Acid-specific Lectin from Hemolymph of the Southeast Asian Horseshoe Crab Tachypleus gigas
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概要
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A lectin was isolated from hemolymph of the Southeast Asian horseshoe crab Tachypleus gigas by using glycophorin HA affinity chromatography and Sephacryl S-300 gel filtration. The purified lectin had a molecular mass of approximately 396kDa and was composed of 13 identical subunits with molecular masses of 31kDa. The serological specificity of the purified lectin was specifically inhibited by sialic acids sialoglycoproteins, but not by neutral sugars, hexosamines, N-acetylhexosamines, or asialoglycoproteins. Although the N-terminal amino acid sequence of the lectin from T. gigas was identical to that from American horseshoe crab (liphemin) by the same purification method and cross reacted with the anti-liphemin serum, the calcium concentration of hemagglutinating activity of the purified lectin showed a smaller optimal concentration than that of liphemin.
- 社団法人日本農芸化学会の論文
- 1993-08-23
著者
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Sato Nobuyuki
Taiyo Central R&D Institute, Taiyo Fishery Co., Ltd.
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Tsuboi Isami
Taiyo Central Research Institute Taiyo Fishery Co. Ltd.
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Matsukawa Masahito
Taiyo Central Research Institute, Taiyo Fishery Co., Ltd.
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Sato Nobuyuki
Taiyo Central R&d Institute Taiyo Fishery Co. Ltd.
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Sato Nobuyuki
Taiyo Central Research Institute Taiyo Fishery Co. Ltd.
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Matsukawa Masahito
Taiyo Central Research Institute Taiyo Fishery Co. Ltd.
関連論文
- Generation of Monoclonal Antibodies for Detection of a Kuchiziro Fish Virus of Tiger Puffer (Takifugu rubripes)
- Isolation and Characterization of a Sialic Acid-specific Lectin from Hemolymph of the Southeast Asian Horseshoe Crab Tachypleus gigas