Immobilization of β-Glucosidase from Scytalidium lignicola on Chitosan

概要

論文の詳細を見る
Chitosan was found to be a better support than alginate beads for immobilization of β-glucosidase from Scytalidium lignicola. The optimum concentration of glutaraldehyde for enzyme immobilization was 0.2%. Immobilized β-glucosidase was more stable in the pH range of 3-6. Immobilized β-glucosidase retained about 70% of its activity at 50℃ after 72 h of incubation while free enzyme lost most of its activity. The log of activity retained vs time was a straight line with free enzyme but was curved for immobilized enzyme. Lineweaver-Burk plots of free and immobilized β-glucosidase gave K_m values of 2×10^<-4> M and 5.5×10^<-4> M for p-nitrophenyl β-D-glucopyranoside, respectively. Addition of immobilized β-glucosidase to a saccharification system gave a 30% increase in reducing sugar availability compared to free enzyme addition and was at least 4 times reusable without appreciable loss in enzyme activity.
社団法人日本生物工学会の論文
1986-06-25