Enzymatic Conversion of Dihydrouracil into Uracil

概要

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We found that some strains of Rhodotorula glutinis can oxidize dihydrouracil to uracil, and we converted dihydrouracil into uracil using the resting and immobilized cells of R. glutinis IFO-0389.The optimum pH of the conversion of dihydrouracil into uracil was 7.8. Oxygen supply was essential to the conversion. With resting cells, the addition of both o-phenanthroline and Triton X-100 caused increase of the yield of uracil about ten times as mush as that with no addition.The conversion ratios of dihydrouracil into uracil using immobilized-cell beads, which were made with chitosan and glutaraldehyde, were 100,98,and 77% when the concentration of dihydrouracil were 1,2,and 3 (w/v) %, respectively, for 68 h at 30℃.
社団法人日本生物工学会の論文
1986-06-25