Effects of Several Factors on CDP-choline Production by Yeast : Enzymatic Studies on Nucleic Acid Related Compounds of Microorganisms (V)

概要

論文の詳細を見る
Favorable reaction conditions were sought for CDP-choline production from CMP and choline compounds by Candida sp. N-25-2. CDP-choline production from CMP and choline was achieved by the addition of toluol to intact cells or dried cells. An examination of the effects of choline compounds on CDP-choline production revealed that phosphoryl choline and choline chloride gave comparably high yields of CDP-choline, but the addition of choline phosphate in high concentration decreased the yield. The effects of other factors, for example, the concentration of glucose, phosphate, Mg^<2+> and intact cells, on CDP-choline production were also studied. Under optimal conditions, a high rate of conversion of CMP to CDP-choline of 80〜87% was obtained. Further, the effect of ATP on CMP phosphorylation and CDP-choline production was investigated in several yeast strains. ATP was found to enhance CTP production but not CDP-choline production.
公益社団法人日本生物工学会の論文
1981-06-25