Isolation and Characterization of a Methanol-assimilating Yeast, Condida methanophilum sp.nov. : Study on the Fermentation of Methanol as a Sole Carbon Source(II).

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A methanol-assimialting yeast was isolated from a sample of soil by enrichment cultivation. On the basis of taxonomical characteristics, it was identified as a new species of yeast and named Candida methanophilum sp.nov. The yeast could grow well on less than 1%(v/v)methanol. The maximum specific growth rate, 0.15(1/hr), was observed at 30℃ and pH 6.5 with periodical feeding of methanol. A biomass of 16.5(g/l)was obtained after 48hr of cultivation. The growth yield for methanol was 36.2%(w/w)which corresponds to 40.0%(w/w)after correction for methanol evaporation during the cultivation. The cell compositon was as follows : crude protein 59.5%, total cellular amino acid 51.6%, extractable lipid 4.6%, crude fiber 2.2%, crude ashes 10.1%, and carbohydrates 22.1%. Pepsin digestability of the yeast was 97.3%. The amino acid profile is also described.
社団法人日本生物工学会の論文
1978-10-25