Specific Oligonucleotide Primers Based on Sequences of the 16S-23S rDNA Spacer Region for the Detection of Burkholderia gladioli by PCR
スポンサーリンク
概要
- 論文の詳細を見る
Specific primers were designed based on the sequences of the spacer region between the 16S and 23S ribosomal DNA (rDNA) for direct, rapid and specific detection of Burkholderia gladioli. These primers were named GLA-f and GLA-r. PCR performed on boiled bacterial suspensions yielded an amplification product of approximately 300 bp. No products from other bacterial species, including B. glumae were amplified, even after complete DNA extraction by the cetyltrimethyl-ammonium bromide (CTAB) method. Using the specific primers designed in this study, the PCR method can detect B. gladioli in plant samples within 6 hr. These data demonstrate the potential of specific PCR for the detection of B. gladioli.
- 日本植物病理学会の論文
- 2002-08-25
著者
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Furuya Naruto
Laboratory of Plant Pathology,Division of Plant Pathology and Pesticide Science,Department of Applie
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TAKESHITA Minoru
Laboratory of Plant Pathology, Division of Plant Pathology and Pesticide Science, Department of Appl
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TAKANAMI Yoichi
Laboratory of Plant Pathology, Division of Plant Pathology and Pesticide Science, Department of Appl
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IIYAMA Kazuhiro
Laboratory of Microbial Control and Insect Pathology, Division of Plant Pathology and Pesticide Scie
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Iiyama Kazuhiro
Faculty Of Agriculture Kyushu University
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Iiyama Kazuhiro
Laboratory Of Insect Pathology And Microbial Control Institute Of Biological Control Faculty Of Agri
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Furuya N
Faculty Of Agriculture Kyushu University
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Furuya Naruto
Laboratory Of Plant Pathology Department Of Applied Genetics And Pest Management Faculty Of Agricult
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URA Hiroyuki
Fukuoka Agricultural Research Center
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URA Hiroyuki
Yame Regional Agriculture Extension Center
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MATSUMOTO Masaru
Laboratory of Plant Pathology, Division of Plant Pathology and Pesticide Science, Department of Appl
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Takada Y
Laboratory Of Plant Pathology Division Of Plant Pathology And Pesticide Science Department Of Applie
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Takanami Yoichi
Laboratory Of Plant Pathology Division Of Plant Pathology And Pesticide Science Department Of Applie
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Matsumoto M
Laboratory Of Plant Pathology Division Of Plant Pathology And Pesticide Science Department Of Applie
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Takeshita M
Kyushu Univ. Fukuoka Jpn
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Takeshita Minoru
Laboratory Of Plant Pathology Division Of Plant Pathology And Pesticide Science Department Of Applie
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Furuya Naruto
Laboratory Of Plant Pathology Division Of Plant Pathology And Pesticide Science Department Of Applie
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