Characterization of Two Cucumber mosaic virus Isolated from Solanum mammosum and Nicotiana affinis

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Two Cucumber mosatic virus (CMV) isolates, CMV-NA from Nicotiana affinis, and CMV-SM from Solanum mammosum (nipple fruit plant) inducing mild and severe systematic mosaic symptoms, respectively, on tobacco (Nivotiana tabacu, cv. Xanthi-nc), were characterized on the basis of their nucleotide and amino acid sequences of RNA3, serology and host ranges. The complete nucleotide sequencing of RNA3s of CMV-NA and CMV-SM has been done. Multiple alignment analysis of the nucleotide sequences of RNA3 of the 5'noncoding region and amino acid sequences of the CP and MP reveased that, CMV-NA and CMV-SM belong to subgroup Ⅰ. CMV-NA induced systematic infection on Cryptotaenia japonica and caused severe leaf malformation, but CMV-SM could not infect this plant. N. occidentalis inoculated mechanically with CMV-SM developed mild mosaic symptoms, while CMV-NA was unable to induce systemic infection on this plant. Thus, C. japonica and N. occidentalis are strain specific hosts for CMV-NA and CMV-SM, respectively. Raphanus sativus cv. Akidumari and Phaseplus vulgaris demonstrated to be non-host plants of CMV-NA and CMV-SM. This is the first report of a CMV strain infecting N. affinis in the field.
九州大学の論文