Kinetics Analysis of Growth and Lactic Acid Production in pH-Controlled Batch Cultures of Lactobacillus casei KH-1 Using Yeast Extract/Corn Steep Liquor/Glucose Medium(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

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• This study was performed to determine the optimal conditions of yeast extract, corn steep liquor and glucose concentration for the growth and lactic acid production of Lactobacillus casei KH-1 and to assess the effect of these conditions using a response surface methodology. A Box-Behnken design was used as an experimental design for the allocation of treatment combination as 17 pH-controlled batch cultures. The growth and product parameters were estimated by Gombertz, Leudeking and Piret models from experimental data, and analyzed statistically with response surfaces. The effects of yeast extract, corn steep liquor and glucose were significant for the maximum specific growth rate, μ_<max>, and the maximum biomass concentration, X_<max>. The interaction of corn steep liquor and glucose indicated that the positive or negative effect of glucose on μ_<max> in corn steep liquor below or above 2.1% could be explained by the glucose-dependent availability of a nutrient on μ_<max>. Although the experiment was achieved in pH-controlled batch culture for L. casei KH-1, the growth- and nongrowth-associated production rate parameters, a and b, were significant in the response surface model. The growth and lactic acid production of L. casei KH-1 were strongly affected by glucose and the importance of the media composition was demonstrated. The estimated optimal conditions of the growth and lactic acid production of L. casei KH-1 were 1.276% and 0.697% for yeast extract, 3.505% and 1.708% for corn steep liquor, and 2.390% and 2.215% for glucose, respectively.

• 社団法人日本生物工学会の論文
• 2003-08-25

著者

• Kim Myong-jun

  Department Of Civil Earth And Environmental Engineering Chonnam National University

• HA MI-YOUNG

  Department of Civil, Earth and Environmental Engineering, Chonnam National University

• KIM SI-WOUK

  Department of Environmental Engineering, Chosun University

• LEE YONG-WOON

  Department of Civil, Earth and Environmental Engineering, Chonnam National University

• KIM SEONG-JUN

  Department of Civil, Earth and Environmental Engineering, Chonnam National University

• Lee Yong-woon

  Department Of Civil Earth And Environmental Engineering Chonnam National University

• Ha Mi-young

  Department Of Civil Earth And Environmental Engineering Chonnam National University

• Kim Si-wouk

  Department Of Environmental Engineering Chosun University

• Kim Seong-jun

  Department Of Adaptive Machine Systems Graduate School Of Engineering Osaka University

関連論文

• Kinetics Analysis of Growth and Lactic Acid Production in pH-Controlled Batch Cultures of Lactobacillus casei KH-1 Using Yeast Extract/Corn Steep Liquor/Glucose Medium(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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