Necessity of a Two-Stage Process for the Production of Azadirachtin-Related Limonoids in Suspension Cultures of Azadirachta indica (PLANT BIOTECHNOLOGY)

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概要

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• The effect of major nutrients on growth and azadirachtin-related limonoids (AZRL) production in plant cell culture of Azadirachta indica (neem) was studied with the objective to increase the yield of AZRL, one of the major group of pesticidal compounds found in intact neem trees. We report the novel online monitoring of plant cell respiration activities in a new parallel shake flask measuring device. Results obtained using three standard plant cell culture media showed non-growth-associated production characteristics for AZRL. These findings were supported by the oxygen uptake rate data. Further investigations on AZRL production in a modified MS medium with different concentrations of nitrogen and phosphorus sources resulted in 0.25 mg・g^1 dry weight of AZRL, compared to no detectable AZRL production in standard MS media. These characteristics suggest the necessity of a two-stage process for the production of AZRL in plant cell culture. Compared to the single-stage process, an almost twofold increase in the volumetric productivity of AZRL was achieved using the two-stage process.

• 社団法人日本生物工学会の論文
• 2003-07-25

著者

• Raval Keyur

  Dept. Of Biochemical Eng. & Biotech. Indian Institute Of Technology Delhi

• Buechs J

  Department Of Applied Chemistry Nagoya Institute Of Technology

• Buchs Jochen

  Department Of Biochemical Engineering Rwth-aachen University

• HELLWIG STEPHAN

  Institute of Molecular Biotechnology, RWTH-Aachen University

• PRAKASH GUNJAN

  Dept. of Biochemical Eng. & Biotech., Indian Institute of Technology Delhi

• RAMOS-PLASENCIA ALFREDO

  Department of BIochemical Engineering, RWTH-Aachen University

• SRIVASTAVA ASHOK

  Institute of Molecular Biotechnology, RWTH-Aachen University

• Ramos-plasencia Alfredo

  Department Of Biochemical Engineering Rwth-aachen University

• Prakash Gunjan

  Dept. Of Biochemical Eng. & Biotech. Indian Institute Of Technology Delhi

• Srivastava Ashok

  Institute Of Molecular Biotechnology Rwth-aachen University

• Hellwig Stephan

  Institute Of Molecular Biotechnology Rwth-aachen University

• BUCHS JOCHEN

  Department of Applied Chemistry, Nagoya Institute of Technology

関連論文

• Necessity of a Two-Stage Process for the Production of Azadirachtin-Related Limonoids in Suspension Cultures of Azadirachta indica (PLANT BIOTECHNOLOGY)
• Solid-Liquid Mass Transfer and Critical Frequency for Complete Suspension in a Reciprocally Shaking Vessel
• Mixing Performance of a Reciprocally Shaking Vessel

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