Neurite Outgrowths of Neurons on Patterned Self-Assembled Monolayers(MEDICAL BIOTECHNOLOGY)
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概要
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The effects of specific chemical functionalities on the neurite outgrowths of embryonic chick dorsal root ganglia (DRG) neurons and PC12h cells were investigated using a set of chemically functionalized surfaces prepared by self-assembled monolayers (SAMs) of alkanethiolates with R=NH_2, COOH, and CH_3 on patterned gold surfaces. The numbers of neurons with neurite outgrowths were compared in the course of a two-week cultivation period. Neurons with neurite outgrowths were observed predominantly on a patterned SAM of long-chain alkanethiolates with amino groups. After about two weeks, the neurons detached from the patterned SAM. However, the activity of β-galactosidase immobilized via a patterned SAM did not decrease over a 13-d period, reflecting the long-term stability of the SAM. Therefore, the neurons became detached upon cell death. These results demonstrate that the patterned SAM of 11-amino-1-undecanethiolate is a scaffold suitable for making cell chips.
- 社団法人日本生物工学会の論文
- 2002-11-25
著者
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Naka Yukie
Bio-Nano Electronics Research Center, Toyo University
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Naka Yukie
Bio-nano Electronics Research Center Toyo University
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Naka Yukie
Faculty Of Life Sciences Toyo University
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Shimizu Norio
Faculty of Life Sciences, Toyo University
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EDA AKIKO
Faculty of Life Sciences, Toyo University
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TAKEI HIROYUKI
Life Science Group, Hitachi, Ltd.
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Shimizu Norio
Bio-nano Electronics Research Center Toyo University:graduate School Of Life Sciences Toyo Universit
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Eda A
Faculty Of Life Sciences Toyo University
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Eda Akiko
Faculty Of Life Sciences Toyo University
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Takei Hiroyuki
Life Science Group Hitachi Ltd.
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Shimizu Norio
Faculty Of Life Sciences Toyo University
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