Epon包埋切片による芽糸球体装置の観察

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There is considerable evidence that the juxtaglomerular apparatus is the site for the production and release of renin. Up to the present, various methods for the staining of the juxtaglomerular apparatus have been reported. But it is difficult to observe the juxtaglomerular apparatus in various forms of diseases definitely in spite of these various methods for the staining. In the present report, we observed the morphology of the juxtaglomerular apparatus by light microscope in epon-embedded 0.5~1μ thick sections, and demonstrated that this method was greatly useful for the observation of the juxtaglomerular apparatus. Method. Renal tissues taken from human subjects and rat were fixed in Bouin solution and embedded in Epon 812 by the method of Luft. The blocks were cut by the ultramicrotome in 0.5~1μ thick sections. After then, the epoxy resin was rernov-ed by the method of Mayor and his associates. The sections were stained with the method of Bowie. Results. In sections of eponembedded tissue, the staining properties by the method of Bowie were similar to those in paraffin sections. Comparing to paraffin sections, as the ipon-embedded sections were cut thinner, the definite appearance of the juxtaglomerular apparatus were observed. Especially the degree of granulality in the juxtaglomerular cells in hyman was observed more clearly than that in paraffin sections. Discussion: Since 1924, when Sugiyama in Japan first described the specific morphology of the wall of the afferent arteriole, the juxtaglomerular apparatus, several staining methods for the juxtaglomerular apparatus have been introduced. Utilizing these staining methods, it has become possible to determine the physiological activities of the juxtaglomerular apparatus, to some extent. But even today, in spite of the improvement of various methods for the staining, it is difficult to observe the degree of granulality in human definitely. The one of the causes seems to depend on the fact that the glomerular vascular pole is not easily found by the previous methods. Then we employed the method of the observation of epn-embedded tissues by light microscope, which 0.5~1μ sections were made and the glomerular vascular pole was easily and clearly found. In addition, in spite of having removed the epoxy resin by the method of Mayor et al., staining properties of the juxtaglomerular apparatus were not different from those in paraffin sections. Therefore, in order to observe the physiological activities of the juxtaglornerular apparatus in human more definitely, this method seems to be more useful than the previous methods. Conclusion. We observed the morphology of the juxtaglomerular apparatus by light microscope in epon-embedded 0.5~1μ thick sections. Juxtaglomerular cells and granules were easily and clearly observed in epon-ernbedded sections rather than in paraffin sections.
社団法人日本循環器学会の論文
1969-06-20

Epon包埋切片による芽糸球体装置の観察

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