ISOLATION AND CHARACTERIZATION OF HUMAN RENIN SUBSTRATE
スポンサーリンク
概要
- 論文の詳細を見る
Human renin substrate (angiotensinogen) was purified from outdated bank plasma. Purification procedures included ammonium sulfate precipitation, DEAE-cellulose column chromatography, concanavalin A-Sepharose column chromatography, Hydroxylapatite column chromatography preparative isoelectric focusing and Ultrogel AcA 44 gel filtration. The final recovery was 10% and the specific angiotensin I content of 10.5 μg/mg of protein was obtained. Polyacrylamide gel and SDS-polyacrylamide gel electrophoresis and analytical ultracentrifugal analyses showed the homogeneity of the purified renin substrate. The molecular weight of 60900 was determined by sedimentation equilibrium studies. Human renin substrate was a glycoprotein containing 13% carbohydrate. Cystine could not be detected on amino acid analysis. The purified renin substrate showed the isoelectric point heterogeneity (pI, 4.6 and 4.9).
- 社団法人日本循環器学会の論文
- 1980-05-20
著者
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Hiwada Kunio
The 2nd Department Of Internal Medicine Ehime University School Of Medicine
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Kokubu Tatsuo
The 2nd Department of Internal Medicine, Ehime University School of Medicine
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SOGO YUKIMI
The 2nd Department of Internal Medicine, Ehime University School of Medicine
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Kokubu Tatsuo
The 2nd Department Of Internal Medicine Ehime University School Of Medicine
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Sogo Yukimi
The 2nd Department Of Internal Medicine Ehime University School Of Medicine
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