[SHORT REPORTS]Meauring β-Endorphin in Cat Cerebrospinal Fluid by a Competitive Enzyme Linked Immunosorbent Assay: The Effects of Surgical Shock and Electrical Stimulation of the Muscular Nerve Afferents

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概要

• 論文の詳細を見る

• We measured β-endorphin in cat cerebrospinal fluid (CSF) using a competitive enzyme linked immunosorbent assay. The amount of antibodies reacting competitively with β-endorphin bound to a solid phase was determined using biotin-anti-IgG and avidin-alkaline phosphatase. The minimal detection limit was approximately 1.5 fmol/well. With this assay systen, β-endorphin-like immnoreactivities (ED-ir) in cat CSF were measured. Postoperative ED-ir levels decreased gradually from 0.68 to 0.24 pmol/ml 3 hr later, while the respiratory rate reciprocally increased. The electrical stimulations of thin-fiber muscular afferents, however, did not induce any consistent changes in ED-ir.

• 名古屋大学の論文

著者

• Kozaki Y

  Department Of Neural Regulation Division Of Regulation Of Organ Function Research Institute Of Envir

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• [SHORT REPORTS]Meauring β-Endorphin in Cat Cerebrospinal Fluid by a Competitive Enzyme Linked Immunosorbent Assay: The Effects of Surgical Shock and Electrical Stimulation of the Muscular Nerve Afferents

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