Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines

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概要

• 論文の詳細を見る

• The cytotoxic and genotoxic effects of goniothalamin, a plant styryllactone, were evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and the Alkaline Comet assay respectively in human leukemic cell lines. Following 72 h of treatment, the IC_<50> values of goniothalamin in human HL-60 promyelocytic leukemia cells and CEM-SS T-lymphoblastic cells were 4.5μg/mL and 2.4μg/mL respectively. The genotoxicity of goniothalamin in both HL-60 and CEM-SS cells was detected as early as 2 h following treatment at IC_<10> and IC_<25> concentrations. However, pretreatment with the antioxidant N-acetyl-cysteine (NAC) at 1 mM for 30 minutes did not abrogate genotoxicity of this compound. This result suggests that primary induction of DNA damage by goniothalamin may not involve oxidative damage. In conclusion, our results demonstrate genotoxic damage induced by goniothalamin in leukemic cells. Further studies are needed to ascertain the mode of action of goniothalamin in inducing DNA damage.

• 日本環境変異原学会の論文
• 2005-11-30

著者

• Rajab Nor

  Department of Biomedical, Universiti Kebangsaan Malaysia

• Rajab Nor

  Department Of Biomedical Science University Kebangsaan Malaysia

• Din Laily

  Faculty Of Science And Technology Universiti Kebangsaan Malaysia

• Hamid Zariyantey

  Department of Biomedical Science, University Kebangsaan Malaysia

• Hassan Hunaizah

  Department of Biomedical Science, University Kebangsaan Malaysia

• Ali Abdul

  Institute of Bioscience, Universiti Putra Malaysia

• Inayat Hussain

  Environmental Health Programme, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia

• Ali Abdul

  Institute Of Bioscience Universiti Putra Malaysia

• Inayat Hussain

  Environmental Health Programme Faculty Of Allied Health Sciences Universiti Kebangsaan Malaysia

• Hamid Zariyantey

  Department Of Biomedical Science University Kebangsaan Malaysia

• Hassan Hunaizah

  Department Of Biomedical Science University Kebangsaan Malaysia

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• Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines
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