Characterisation of Bifidobacterium Species-A Review

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概要

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• Identification of Bifidobacterium species are a difficult task because of phenotypic and genetic heterogeneities. Various DNA-based techniques to rapidly characterise Bifidobacterium species and to support the conventional biochemical and morphological classification methods have been described. Sequencing of the 16S rRNA gene and 16S to 23S internally transcribed spacer region and comparing with the sequences data present in GenBank are the most popular techniques in identifying Bifidobacterium species. Conserved sequences other than the 16S rRNA gene such as ldh, recA and hsp60 genes have become worthy tools for the elucidation of various taxonomic features such as genera, species and strains of Bifidobacterium. However, as an alternative to sequencing which is both time consuming and technically demanding, genus- or species-specific primers or probes were successfully designed to rapidly identify Bifidobacterium species. In this review, amplified ribosomal DNA restriction analysis (ARDRA) method derived from the 16S rRNA gene is also discussed because of it rapid, reproducible and easy to handle characteristics. Furthermore, randomly amplified polymorphic DNA (RAPD), Pulsed-Field Gel Electrophoresis (PFGE) and repetitive elements fingerprinting (Rep) were the popular methods to study the genetic diversity among Bifidobacterium species due to its versatility.

• 日本ビフィズス菌センターの論文
• 2004-04-01

著者

• Saleh Norihan

  Department Of Biotechnology Faculty Of Food Science And Biotechnology Universiti Putra Malaysia

• SHUHAIMI Mustafa

  Department of Food Technology, Faculty of Food Science and Biotechnology, Universiti Putra Malaysia

• Ali Abdul

  Institute Of Bioscience Universiti Putra Malaysia

• Yazid Abdul

  Department Of Food Technology Universiti Putra Malaysia

• NORJIHAN Alitheen

  Department of Biotechnology, Universiti Putra Malaysia

• Shuhaimi Mustafa

  Department Of Biochemistry And Microbiology Universiti Putra Malaysia

• Norjihan Alitheen

  Department Of Biotechnology Universiti Putra Malaysia

• SHUHAIMI Mustafa

  Department of Biochemistry and Microbiology, Universiti Putra Malaysia

関連論文

• Evaluation of the cytotoxic and genotoxic effects of goniothalamin in leukemic cell lines
• Classification of Bifidobacterium Isolates from Infant Faeces Using PCR-Based and 16S rDNA Partial Sequences Analysis Methods
• Differentiation of the Bifidobacterium pseudocatenulatum Isolates from Faeces of Infant by RAPD
• Characterisation of Bifidobacterium Species-A Review
• Strain Differences in Deconjugation of Bile Acids in Bifidobacterium pseudocatenulatum Isolates

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