Conversion of Lactate to Methane by Triculture of a Sulfate Reducer and Methanogens

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概要

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• Complete conversion of lactate to methane and CO_2 by triculture with a sulfate reducer, strain F-1,a hydrogen-utilizing methanogen, strain HM-1,and an acetate-utilizing methanogen, strain AM-1,was studied with sulfate-free medium under N_2 and Co_2. Strain F-1 was motile, Gram-negative and vibrio-shaped cells which utilized H_2,lactate and ethanol as electron donors. Strain F-1 were hybridized with the oligodeoxynucleotide probe complementary to specific regions of mesophilic Gram-negative sulfate-reducing bacteria (SRB probe). The G+C content of the DNA was 63.2 mol%. Strain F-1 was tentatively classified into Desulfovibrio vulgaris. Strain F-1 was unable to degrade lactate in the sulfate-free medium under N_2 and CO_2. However, it degraded lactate in the dual culture with strain HM-1 in sulfate-free medium. Lactate was completely converted to methane and carbon dioxide by triculture with strain F-1,strain HM-1 and strain AM-1 which was a filamentous acetate-utilizing methanogen. The triculture formed flocs and grew well on the bottom of culture flasks. Acetate accumulated in the dual culture was used concurrently with its production in the triculture. Acetate conversion to methane by strain AM-1 was not inhibited by hydrogen. Lactate was converted to methane and CO_2 stoichiometrically by the triculture in a sulfate-free minimal medium. The 16S rRNA-targeted oligodeoxynucleotide fluorescent probe in situ hybridization method was used for identification and enumeration of strain F-1 in the microbial triculture ecosystem. A large number of strain F-1 cells hybridized with the SRB probe were observed while lactate was consumed at the early stage of incubation.

• 日本微生物生態学会の論文
• 1995-05-31

著者

• TAKEDA Kiyoshi

  Faculty of Agriculture and Life Science, Hirosaki University

• Fukui M

  National Institute For Resources And Environment

• Fukui Manabu

  National Institute For Resources And Environment Aist Miti

• Takeda K

  Faculty Of Agriculture Hirosaki University

• Takeda Kiyoshi

  Faculty Of Agriculture And Life Science Hirosaki University

関連論文

• Characterization of cultivable methanotrophs from paddy soils and rice roots(Soil Biology)
• Characteristics of an Anaerobic, Syntrophic, Butyrate-degrading Bacterium in Paddy Field Soil(Microbiology & Fermentation Technology)
• Major substrates for microbial sulfate reduction in the sediments of Ise Bay, Japan
• Distribution of Methanotrophic Bacteria in the Coastal Marine Sediments
• Characteristics and Propionate Production of Propionibacterium Isolated from a Methane fermentation Digester
• Conversion of Lactate to Methane by Triculture of a Sulfate Reducer and Methanogens
• Microdistribution of sulfate-reducing bacteria in sediments of a hypertrophic lake and their response to the addition of organic matter

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