ウシ副腎皮質細胞におけるコルチコイド産生に対する小青竜湯の影響

概要

論文の詳細を見る
We examined the effects of Sho-seiryu-to on the corticoidogenesis (CG) in primary cultured bovine adrenocortical cells. In order to evoke CGs, adrenocorticotropic hormone (ACTH), dibutyryl cyclic AMP (db-cAMP) which mimics intracellular action of cAMP, or 30mM K^+ which activates Ca^<2+> channel with a voltage-dependent manner were used as stimulants. Primary 72h-cultured cells were incubated with Sho-seiryu-to under the presence of the stimulants at 37℃ for 1h. Both 100 pM and I nM ACTH-induced CGs increased at either low (10^<-6>g/ml) or high (10<-3>g/ml) concentration of Sho-seiryu-to, but 30mM K^+-induced CG was affected at neither of them. On the other hand, both 0.5 and 2mM db-cAMP-induced CG increased at high concentration of Sho-seiryu-to, but not at low. These results indicate that Sho-seiryu-to, influencing cAMP formation, enhances ACTH-induced CGs and that it stimulates, at a high concentration, the hormone production process beyond cAMP formation.
和漢医薬学会の論文
1999-10-15