G214 マイクロチップによるバイオ分子のラチェット式電気泳動(オーガナイズドセッション11 : マイクロ熱流体システム)

概要

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A ratcheting electrophoresis microchip (REM), a novel microdevice that can separate biomolecules such as DNA and proteins in aqueous solution using low applied voltages as low as 1 V, is introduced in the present report. The device consists of several thousands of parallel linear electrodes with a pitch of 10 μm typically, which is produced easily by the microlithography technique. A spatial saw-tooth potential distribution is generated by the electrode array, which works to drive charged analyte molecules to migrate in the electrophoresis fashion. Results of simple 1-D simulations suggest the REM can function as a molecular filter through which only molecules having mobility larger than a desired threshold can pass. The REM can also be programmed to separate molecules to create a molecular profile, much like conventional electrophoresis. 2-D simulations for a real device, which consider molecular diffusion, transient development of diffuse-electric double layers near the electrodes as well as electrophoretic migration of analyte molecules, are also performed and feasibility of this chip is proved.
2001-11-03