Enzyme Linked Immunosorbent Assayによるアニサキス幼虫粗抗原特異IgE抗体の検出

概要

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Semi-determination of Anisakis larvae crude antigen specific IgE in human serum was performed by the method of enzyme linked immunosorbent assay (ELISA) in order to study the relationship between anisakiasis and anaphylaxis. Using the Ouchterlony method, significantly higher values of specific IgE were obtained from sera with precipitin bands to Anisakis larvae crude antigen than from control sera. Especially high values were obtained from sera of anisakiasis. To study the role of crude antigen for coating in ELISA in immunodiagnosis, crude antigen was fractionated into six parts on Sephadex G-200 column. Antigen determinant fraction of sera from five patients was investigated by ELISA. As a result, the antigen determinant fractions were showed difference with individual sera.
日本アレルギー学会の論文
1985-07-30