結核死菌感作モルモットにおけるMiddlebrook-Dubosの間接的赤血球凝集反応及び溶血反応の19S及び7S抗体活性
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By observing that there was no obvious relation between the hemagglutination titer in the Middlebrook-Dubos indirect hemagglutination test and the hemolytic titer in the hemolytic modification of the test, the duality of antibody responsible for these two reactions has been discussed repeatedly. Some attempts to separate each antibody have not satisfactorily made, by using paper electrophoresis and DEAE-celllose column chromatography. In order to approach this problem from other aspect, the author attempted to see if the two serologic reactions could dissociate in the course of 19S and 7S antibody response. The serum of guinea pigs was fractionated by Sephadex-G-200 gel filtration at 2, 4, 6, 11, and 16th week after the sensitization with killed tubercle bacilli suspended in paraffine oil. Antibodies included in the first two major fractions of the chromatogram was confirmed to have sedimentation coefficients of 19S and 7S, respectively, by using ultracentrifugal and sucrosd density gradient analysis and sensitivity to 2-mercaptoethanol. The hemagglutination and hemolytic activities were observec to appear and proceed independently from each other in the two fractions, early in 19S ane late in 7S fractions as indicated below. In combination with the above analysis, starch zone electrophoretic studies indicated that these fast and slow sedimentating antibodies were 1gM and 1gG, respectively. 1) The hemagglutination reaction could be observed early only in the 19S class antibody fraction ahead of the hemolytic reaction, being maintained for a longer period of time until the 16th week in this macromolecule fraction. On the other hand, the hemagglutinating activity in the 7S-fraction was detected at 11th week after the sensitization still in low titer. 2) The hemolytic activity appeared first at 4th week in the 19S fraction and then rapidly transfered to 7S fraction at 6th week. After 11th week no hemolytic activity was detected in the 19S fraction, while the activity in higher titer was found in the 7S fraction. The results can be interpreted as evidence that the antibodies which are measured in the two reactions are different.
- 日本アレルギー学会の論文
- 1967-04-30
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- 結核死菌感作モルモットにおけるMiddlebrook-Dubosの間接的赤血球凝集反応及び溶血反応の19S及び7S抗体活性