蛍光抗体法を応用した腫瘍組織抗原性の研究
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概要
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As an approach to the immunological studies of cancer, the author investigated the antigenicity of subcellular fractions of tumor cells by fluorescent antibody technic. Antisera to each subcellular fractions of Ehrlich ascites tumor cells and of AH 130 cells were made in rabbits, and besides, 3 fractions prepared from nuclear fraction of AH 130 were also used in immunisation. Using these antisera, tumor cell specificity was investigated by precipitation, complement fixation test, and fluorescent antibody staining. As the control organ, mouse liver for Ehrlich tumor cells, and rat liver for AH130 cells were used and the staining properties of antisera after absorption were examined. Following conclusions were obtained from above experiments. 1) Concerning Ehrlich ascits cells, relatively tumor specific antigenicity were found in nuclear and microsomal fractions. Specific fluorescent staining of tumor cells was obtained, after absorption of antisera by mouse liver, but staining was weak. 2) Concerning AH 130 cells, relatively specific tumor antigenicity by CFT was obtained in nuclear fraction, acid protein component, insoluble fraction, and microsomal fraction. By fluorescent antibody staining, the highest tumor cell specificity was found in microsomal fraction and the highest staining titers was found in anti-insoluble fraction. But by investigation of cross reaction to normal organs and tissues, these cross reactions to some tissues, esp. connective tissues were not completely excluded. 3) The same investigation was made in human gastric cancers, but clear cancer cell specific fluorescent stain was not obtained in common among difterent individual materials, except in one case, in which cancer cell were clearly stained by antiserum to difterent materials. 4) Antiserum prepared by normal human gastric mucosa, stains normal human gastric surface epithelium, but this staining was negative by anti-cancer sera. Loss of some antigenicity in cancer was suggestive.
- 日本アレルギー学会の論文
- 1965-07-30