アレルギー機序におけるプラスミンの態度について
スポンサーリンク
概要
- 論文の詳細を見る
Plasmin was prepared from the blood of various animals in different ways and injected it into guinea pigs whether it produces the shock or not. Plasmin was added to various animal's blood in vivo and in vitro and tested whether cytolysis developed or not. The results obtained are as follows; 1) When plasmin from various animals was injected intravenously into guinea pigs, anaphylactoid shock developed in same species, but did not in heterospecies. 2) Leucolysis by plasmin was observed. Plasmin (supernatant of 5,000 r.p.m. centrifugation) prepared from various animals seemed to develop the leucolysis. However, no leucolysis developed with plasmin (supernatant of 1,000 r.p.m. centrifugation). 3) After injection of human plasmin or heated human plasmin intravenously into guinea pigs, the blood platelets counts were performed. The highest decrease of platelets was observed at 5 minutes after the injection and the decrease was observed at 60.1% in non-heated human plasmin and 49.4% in heated human plasmin. 4) The release of histamine-like substance from isolated rat mast cells by plasmin was tested. The releasing percentages by antigen were 38.5%, and by heated plasmin 17.8%. 5) The morphological changes of mast cells after adding plasmin were observed by toluidine blue supravital stainings. The cells were counted at five minutes after stainings. Percentages of ghost cells in all mast cells were 32.0% in plasmin, 20.6% in heated plasmin and 1.2%. in control. It appears from these observations that plasmin which is activated by the antigen-antibody reaction, attacks the mast cells and blood platelets, then histamine and other mediators are released. Consequently, the plasmin might has some role in the anaphylactic shock or allergic reaction.
- 日本アレルギー学会の論文
- 1962-06-30