Effects of 2-Deoxy-D-glucose on DNA Repair and Mutagenesis in UV-irradiated Yeast

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We have studied the effects of 2-deoxy-D-Glucose (2-DG) on the recovery of potentially lethal damage (PLDR), repair of chromosomal DNA, cyclobutane pyrimidine dimers (CPDs), reverse mutation and gene-conversion in UVC (254 nm) irradiated yeast. As studied by pulsed-field gel electrophoresis, post-irradiation chromosomal DNA repair kinetics in a phosphate buffer (PB) with 10 mM glucose (G) was biphasic, where the first phase exhibited a decrease and the second phase showed an increase in the band intensities. A post-irradiation treatment in PB+G (10 mM) with 2-DG (10, 20, 50 mM) reduced the decrease in the DNA band intensities in the first phase of DNA repair. As compared to a post-irradiation (125 J/m^2) treatment in PB+G (10 mM), a treatment in PB+G (10 mM) + 2-DG (10 mM) showed a decreased PLDR, but increased revertants and gene-convertants.
日本放射線影響学会の論文