Adenylate Cyclase-the More Membrane Associated, the Less Radiosensitive

概要

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A dose dependent but not parallel decreases were observed both in SH content and catalytic activity of "free" catalytic subunit after irradiation (0-3200 Gy), while SR groups of membrane-associated adenylate cyclase were insensitive (under 3200 Gy). An initial "radioactivation" of membrane-associated enzyme was found under 800 Gy, then an inhibition above 1600 Gy. The SH alkylating agent, N-ethylmaleimide resulted in a complete inactivation, both of membrane associated form of adenylate cyclase and "free" catalytic subunit with similar inactivation profiles. These data indicate that in the radiosensitivity or "radioprotection" of adenylate cyclase, its membrane association / integration might play a more important role than the SR groups themselves. Radiation modification of plasma membranes and membrane associated components seems to be more complex, than simple peroxidation of membrane lipids and/or oxidation of SH groups of membrane proteins1, 2).
日本放射線影響学会の論文