Radiation-Induced Fluorescence Changes of 2'-Deoxycytidine and Cytidilyl-Cytidine in Aqueous Solution. Comparison with Radiolytic Behaviors of Other Nucleobase Derivatives

概要

論文の詳細を見る
We have reported previously on the radiation-induced formation of highly fluorescent products from thymine, cytosine, adenine, 2'-deoxyadenosine, and 2'-deoxyguanosine. For comparison with these substances, 2'-deoxycytidine (5.0 × 10^<-4> mol/dm3) and cytidilyl-cytidine (2.5 × 10^<-4> mol/dm^3) in buffered aqueous solution (pH 7.0) were irradiated with ^<60>Co gamma-rays under various atmospheric conditions. Fluorescence yield increased markedly with increase of radiation dose under O_2, N_2 and N_2O but not under t-butanol-N_2, indicating that OH radical is the predominant participant in the formation of highly fluorescent products and that O_2 does not suppress the yield. These behaviors are very similar to those for cytosine. However, the fluorescent products from 2'-deoxycytidine and cytidilyl-cytidine were radiolytically more stable than those from cytosine. To summarize the formation of highly fluorescent products from various kinds of nucleobase derivatives by radiatioh, the order for fluorescence yield can be arranged as follows:C >A >G >T under O_2, A >G >C >T under N_2O, and C >A >C >T under t-butanol-N_2, excepting the complex yield under N_2.
日本放射線影響学会の論文