Comparison of the Electrophoretic Method with the Sedimentation Method for the Analysis of DNA Strand Breaks
スポンサーリンク
概要
- 論文の詳細を見る
Application of electrophoresis to the analysis of DNA strand breaks was studied comparing with the sedimentation analysis. A BRL gel electrophoresis system (Type V16) was used for this study. Calf thymus DNA (1 mg/ml) irradiated with ^<60>Co gamma-rays in SSC solution was applied to both the electrophoretic analysis and the sedimentation analysis. Lamda phage DNA and its fragments were employed as the standard size molecules. In a range from 1 k base pairs to 6 k base pairs in length for double stranded DNA or from 2 k bases to 12 k bases for single stranded DNA, the calculated average molecular weight from the electrophoresis coincided with that from the sedimentation. Number of single strand breaks and double strand breaks were 1.34 x 10^<11> breaks/mg/rad (G = 0.215) and 0.48 x 10^5 breaks/mg/rad^2, respectively. A number of papers have been published on the strand breaks in DNA induced by radiation exposure. Since the report of McGrath and Wihiamsl), sedimentation analysis by ultracentrifugation has been used as a standard technique in investigating the DNA strand breaks. Before the introduction of sedimentation analysis, viscosity measurement and light scattering have been used.
- 日本放射線影響学会の論文
著者
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Hoshi Masaharu
Research Institute For Nuclear Medecine And Biology Hiroshima University
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Ogawa Masaaki
Research Center For Odontology School Of Life Dentistry At Tokyo The Nippon Dental University
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Ogawa Masaaki
Research Institute For Nuclear Medicine And Biology Hiroshima University
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YAMAMOTO OSAMU
Research Institute for Nuclear Medicine and Biology, Hiroshima University
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Yamamoto Osamu
Research Institute For Nuclear Medicine And Biology Hiroshima University
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HOSHI MASAHARU
Research Institute for Nuclear Medicine and Biology, Hiroshima University
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