Ultraviolet Light Sensitivity and Unscheduled DNA Synthesis in Various Cell Strains Derived from C57BL Mouse

概要

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Relationship between levels of ultraviolet light (UV)-induced unscheduled DNA synthesis (UDS) and sensitivities to cell killing action of UV was examined, using various mouse cell lines. The level of UDS did not correlate with UV sensitivity in terms of D_O values in dosesurvival curves. This result suggests that UDS as measured by a routine method in mouse cell may reflect other factors than repair capacity. Difference in genome size among cells was suggested, as one of the possible factors, to affect "apparent UDS capacity", by using the DNA microfluorometry combined with autoradiography. Unscheduled DNA synthesis, UDS, as measured by incorporation of ^3H-thymidine into DNA of cells after treatment with radiation or chemicals, has been generally used as a convenient indicator of excision repair of damages induced on DNA. For example, the measurement of UDS has been proposed, and used as one of the possible short term tests for mutagenecity screening.
日本放射線影響学会の論文