メダカ特定座位突然変異 : 発生過程での生殖細胞突然変異の検出
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概要
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A specific-locus test(SLT)system has been established using the fish medaka(Oryzias latipes), where recessive visible mutations(at the b, lf or gu loci)detected during embryonic stages(TM)or after hatching(VM), and dominant lethals(DL)can be examined in the same individual F_1 progeny of treated parents. It was found that about 90% of the F_1 embryos with γ-ray-induced specific-locus mutations were concomitantly accompanied by DL irrespective of doses and germ-cell stages, suggesting that DNA alterations in such mutants might include both either one of the marker loci and region(s)responsible for DL. In contrast, embryonic lethality of ENU(ethylnitrosourea)-induced specific-locus mutants varied with the different concentrations and germ-cell stages treated. DNA alterations at the 87 AP-PCR markers were examined for individual dominant lethal embyros from 4.75 Gy-irradiated sperm or spermatids and the results indicated that such embryos had multiple DNA deletions in the genome. To examine whether irradiated male germ cells manifest genetic changes after fertilization, we began to investigate mosaic mutations at the wl locus during embryogenesis. Wildtype inbred HNI males were irradiated with Cs-137 γ-rays and mated to non-irradiated newly established T5(b/b;lf/lf;gu/gu;i^b/i^b;wl/wl)females. When sperm or spermatids were irradiated, a dose-dependent increase in the frequency of mosaic mutant at the wl locus was observed.
- 日本環境変異原学会の論文
- 1998-07-21
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