N-Formamido Group Formed in the Place of Thymine Fragment under Gamma lrradiation of DNA. Rapid Indirect Mcthod of Evaluation both in vitro and in vivo Conditions

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概要

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• N-formamido-(DNA) is one of the main lesions formed on the thymidylic fragments of DNA when DNA, in vitro or in vivo conditions, is exposed to ionising radiations. The N-formamido group partly replaces the thymine base in DNA sequence. This article proposes an indirect dosing technique for this damage. The method is based on the release of ^<14>CO_2 from the C-2 of the thymine ring. When the pyrimidine ring of the thymidine moiety is broken, N-formamido deoxyribose derivative, carbon dioxide and pyruvamide are formed all in the same reaction. During this investigation the phenomena of protection due to the constituent parts of the cell and to the secondary and tertiary structures of DNA are also evidenced. Although the action of ionising radiations on DNA is now well understood for its chain cleavage, this is not the case for the breaking of the bonds on the constituent parts of the double helix in spite of the extent of work donen. The radiosensitive thymine fragment has been studied to greatest extent, and research on the free base, the nucleoside, the nucleotide and dTpT has permitted the determination, in vitro, of the different lesions suffered by the thymine fragment when DNA is exposed to ionising radiations. In vivo, recent studies have elucidated the elimination of thymine and thymidine following irradiation of bacterial cells. Similarly assay techniques have been developped for products of the 5, 6 dihydroxy-dihydrothymine type and for damage to the thymine methyl groups.

• 日本放射線影響学会の論文

著者

• Teoule R.

  Laboratoire De Radiobiochimie Departement De Recherche Fondamentale Centre D'etudes Nucleaires

• BONICEL A.

  Laboratoire de Radiobiotogie, Department de Recherche Fondamentale, Centre d'Etudes Nucleaires de Gr

• Bonicel A.

  Laboratoire De Radiobiochimie Departement De Recherche Fondamentale Centre D'etudes Nucleaires

関連論文

• Gamma-Irradiation of Cytosine ^C-2 Labelled DNA of Eschedchia coli Identification of the Major Radiolysis Products Resulting from Rupture of the N-Glycosidic Bond
• N-Formamido Group Formed in the Place of Thymine Fragment under Gamma lrradiation of DNA. Rapid Indirect Mcthod of Evaluation both in vitro and in vivo Conditions

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