Effects of Cobalt-60 Gamma Rays on Lysozyme in Aqueous Solution (II)

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概要

• 論文の詳細を見る

• Egg-white lysozyme was irradiated in 0.5 % aqueous solution with cobalt-60 γ-rays. The resulting samples were fractionated by gel-filtration of Sephadex G-100 and treated with mercaptoethanol in order to clarify the polymerization caused in radiation-inactivated lysozyme. Gel-filtration pattern of irradiated lysozyme revealed two protein fraction. The first peak of them showed no enzyme activity and higher molecular weight than intact lysozyme. The second corresponded to intact lysozyme. Mercaptoethanol-reduction studies showed the conversion of inactive polymerized component of irradiated lysozyme into a form derived from intact lysozyme by this treatement. These evidences suggest that a large part of inactivation of lysozyme by p-ray irradiation proceeds in a polymerization caused by intermolecular disulfide bond and the polymerized component is responsible for radiation-inactivation of lysozyme.

• 日本放射線影響学会の論文

著者

• 山本 和彦

  Laboratory Of Radiobiology Department Of Biology Faculty Of Science Osaka University: (present Addre

関連論文

• Effects of Cobalt-60 Gamma Rays on Lysozyme in Aqueous Solution (II)
• Effects of Cobalt-60 Gamma Rays on Lysozyme in Aqueous Solution

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