Effects of Cobalt-60 Gamma Rays on Lysozyme in Aqueous Solution

概要

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Lysozyme in 0.5% aqueous solution has been irradiated with several doses of Co^<60>-γ-rays. The effects of γ-radiation on lysozyme molecule have been studied by the methods of amino acid analysis, N-terminal amino acid analysis, measurement of amide-nitrogen content, ultraviolet spectroscopy, and ultracentrifugal analysis. In sample losing 69% of its initial enzyme activity on irradiation of 1.4×10^6 R. , no significant change is observed in amino adid composition and amide-nitrogen content. And any other N-terminal amino acids than lysine which is expected to be released by peptide bond cleavage, are not detected. But, ultracentrifugal analysis of the irradiated lysozyme indicates the existence of polymerized component in addition to the intact lysozyme. Mechanisms of γ-ray inactivation on lysozyme and roles of the polymerized component have been discussed.
日本放射線影響学会の論文